Medical Research Council - London Institute of Medical Sciences

Now in our 12th year of bringing you beautiful imagery from biomedical science every day

In the Details

3D wide-field fluorescence microscopy enables detailed imaging of brain blood vessels

21 January 2023

In the Details

When it comes to seeing the bigger picture, wide-field fluorescence microscopy is a technique often used by researchers. It allows them to image entire tissues in action. However, it isn’t so great at capturing the finer details. Researchers now present a solution for capturing high-resolution 3D images of live whole tissues – volumetric wide-field fluorescence microscopy combined with two established microscopy techniques called optical astigmatism and fluorescence source localisation. Using this system, researchers captured real-time, high-resolution images of blood vessels (pictured, left) and blood flow (right) in live mouse brains, with colours depicting varying depths within the tissue and blood flow velocity, respectively. When strokes were induced in these mice, this system captured changes in the shape and function of the brain blood vessels, demonstrating the potential of this approach for investigating disease in real time and in fine detail.

Written by Lux Fatimathas

Search The Archive

Submit An Image

What is BPoD?

BPoD stands for Biomedical Picture of the Day. Managed by the MRC London Institute of Medical Sciences the website aims to engage everyone, young and old, in the wonders of biomedicine. Images are kindly provided for inclusion on this website through the generosity of scientists across the globe.

Read More

BPoD is also available in Catalan at with translations by the University of Valencia.